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Preimplantation genetic diagnostics at Lifeline Clinic

Preimplantation Diagnostics is the guaranty of your baby’s genetic health

Preimplantation genetic diagnostics

Preimplantation genetic diagnostics (PGD) is a modern method of diagnostics of genes/chromosomes of embryos produced with IVF technique. The analysis is performed in embryology laboratories with various methods having one and the same purpose, i.e. to identify healthy embryos without chromosomal anomalies prior to their transfer to a female body. Preimplantation genetic diagnosis (PGD) reduces by many times the risks of becoming pregnant with a fetus having genetic abnormalities and chromosome mutations down to minimal risks even for the couples who have hereditary problems with a genetic code.

PGD in IVF allows to identify violations of the chromosome set (aneuploidy) and avoid their planting. Such pathologies, if missed, lead either to miscarriage or (if fatal) to fetal death or to the birth of a baby with one of the following syndromes: Down’s, Edward’s or Patau’s syndrome. Preimplantation prenatal diagnostics is the ideal way to avoid these problems, saving energy and time for raising a healthy child.

Why you should go to Lifeline Clinic

Life Line Reproduction Center performs IVF with PGD, identifying over 250 diseases caused by genetic disorders. A PGD method is selected by a geneticist individually for each couple. Before PGD procedure, a couple has a consultation with a geneticist. There are a number of good reasons to choose our Center:

Innovative expert equipment at our Clinic allows you to choose any
modern methods for a prenatal diagnosis. This is important because each couple has its own threshold
of the disease risk.

The analysis accuracy is due to
the high level of knowledge and experience of our specialists. The number of children born with genetic abnormalities after IVF with
PGD is zero.

Ability to determine gender identity.
This is important in case of hereditary diseases transmitted by male
or female line only.
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+7 495 668 6171

Timely visit to a medical specialist increases the chance of the pregnancy onset by several times

When you are to resort to preimplantation genetic diagnostics

This procedure is indicated in the following cases:

  1. a genome pathology is present in the karyotype of one or the both partners, bearing the risk of pathology transmission to offsprings;
  2. if a woman has a history of spontaneous or missed abortions;
  3. two or more failed IFV attempts;
  4. If a woman has the age over 35, and/or a man is over 40 (the number of genetic abnormalities in germ cells increases with age).

Results of PGD in IVF

This procedure enables a lot of things:

  1. to identify embryos with a normal genotype in case a family has hereditary genetic and chromosomal abnormalities (balanced chromosomal translocations, monogenic diseases);
  2. to reduce the number of IVF procedures in cases where failures were due to genetic anomalies in the embryo. Multiple karyotype anomalies are found in the most of embryos if they are obtained from women over 35 or from patients with adverse IVF outcome (impaired spermatogenezis, more than three IVF/ICSI attempts, more than three IVF attempts);
  3. to find for a sick child a donor who is absolutely identical by leukocyte antigen (brother/sister) is a method of HLA typing of embryos;
  4. to identify embryos prone to late manifestation and having a genetic predisposition to the most severe diseases (Alzheimer's disease, cancer tumors, etc.);
  5. to determine the gender identity of an embryo in order to avoid transfer of diseases transmitted by certain lines.

Specifics of PGD procedure at Life Line Center

The procedure is performed during cultivation of embryos. The geneticist takes one or more cells, depending on a PGD method, from each sample. Then, the resulting material is subjected to the genetic analysis. Since during preimplantation development stages all embryo cells are identical, the specialist can make conclusions about the complete genome of the embryo. An erroneous result is possible in the study of embryos with heterogeneous genetic composition of cells, which is very rare.

Biopsy procedure may be performed on various dates.

  • Biopsy of blastomeres is performed at the embryo-morula stage when an embryo has only 6-8 cells. One of the cells, called blastomere, is carefully sampled using innovative micro-manipulation equipment, then fixed in a certain way and sent to the genetic laboratory where studies are performed.
  • Trophectoderm biopsy is performed at a later stage of blastocyst embryos when an embryo is 5 days’ old. The specialist takes several cells from a trophoblast. Later on, extraembryonic membranes will be formed from this part of the embryo.
  • These procedure options are safe for embryo development, if the procedure is properly performed.

    The analysis of cells obtained during biopsy can be performed using the two methods:

    1. fluorescent hybridization in situ (international designation – FISH);
    2. comparative genomic hybridization (international designation – CGH).

    These methods are suitable for biopsy of blastomeres and trophectoderm.

    The geneticist marks specific chromosomes with fluorescent dyes and then performs a microscopic analysis of the sample with a special-purpose microscope. This method is less expensive than the other PGD methods, but it is limited in chromosome detection opportunities. Usually, FISH analysis is performed on an aneuploidy by 5 chromosomes: 13, 18, 21, X, Y, or 9 chromosomes: 13, 15, 16, 17, 18, 21, 22, X, Y.

    When PGD is performed at the blastomere stage (day 3 of development), information on the genetic status of all embryos becomes available within 1-2 days. Next, on Day 5 of cultivation, healthy embryos are selected with subsequent transfer during the fresh cycle. During trophectoderm biopsy, healthy embryos are subjected to cryoconservation with cryotransfer during one of the cycles.

    The price of preimplantation genetic diagnosis

    When you study the list of Moscow centers that offer PGD procedure, you will see that the cost of this procedure is quite high. You must understand that this is due to good equipment of the laboratories – it is an obligatory requirement for performance of PGD, ICSI, and IVF procedures. Besides, PGD's price depends on the method chosen.

    Geneticists often recommend the trophectoderm biopsy method. This is due to the ability of getting two and more cells for the test, which respectively increases the analysis accuracy, since the sample has a greater number of DNAs. Besides, this technique is often cheaper for patients, since it is performed on Day 5 of cultivation, when the accuracy of assessment of embryo potential enables selection of the most promising embryos, and use only them in the preimplantation genetic diagnosis procedure.

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